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ErpA8 Antibody

Erpn/Ospe antibody

Product Specifications

Product Name Alternative

Rabbit anti-ErpN Antibody, rabbit anti-OspE Antibody, rabbit anti-ErpN/OspE Antibody, ErpA, outer surface protein E, Borrelia burgdorferi OspE, ospE/F-Related Protein N, ErpA8, BB_L39, cp32-8

UniProt

H7C7N5

Reactivity

Bacteria

Immunogen

MBP-fusion protein corresponding to Borrelia burgdorferi ErpN/OspE protein.

Target

ErpA8

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

1.1 mg/mL

Dilution

ELISA: >1:5,000, WB: 1:1,000

Purity

This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. It is directed against, and shows specific reactivity for, Borrelia burgdorferi OspE protein. Reactivity with ErpN/OspE protein from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-ErpN/OspE antibody has been tested in ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at 17.1 kDa in size corresponding to ErpN/OspE by Western blotting in the appropriate cell lysate or extract.

Tested Applications

ELISA, WB

NCBI Accession Number

WP_010883865.1

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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