DbpA, BB_A24 Antibody

This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi DbpA protein. A BLAST analysis was used to suggest cross-reactivity with DbpA from Borrelia burgdorferi sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against Borrelia garinii sources based on 60-80% homology. Cross-reactivity with DbpA from other sources has not been determined.

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

This protein-A purified antibody has been tested for use in ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 18 kDa in size corresponding to Borrelia burgdorferi DbpA protein by Western blotting in the appropriate cell lysate or extract.