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RUB1 Antibody

Rub1 antibody

Product Specifications

Product Name Alternative

Rabbit anti-Rub1 antibody, NEDD8-like protein RUB-1, Ubiquitin-like protein RUB 1

UniProt

Q03919

Reactivity

Yeast

Immunogen

Rub1 Antibody was prepared from rabbit serum after repeated immunizations with full-length recombinant yeast Rub1 protein.

Target

RUB1

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

1.0 mg/mL

Dilution

ELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,000

Purity

Rub1 Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Rub1 Antibody is tested in western blot and defects a 6 kDa band corresponding to yeast Rub1. Most yeast cell lysates can be used as a positive control without induction or stimulation.

Tested Applications

ELISA, WB

NCBI Accession Number

Q03919.1

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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