RUB1 Antibody
Rub1 antibody
Product Specifications
Product Name Alternative
Rabbit anti-Rub1 antibody, NEDD8-like protein RUB-1, Ubiquitin-like protein RUB 1
UniProt
Q03919
Reactivity
Yeast
Immunogen
Rub1 Antibody was prepared from rabbit serum after repeated immunizations with full-length recombinant yeast Rub1 protein.
Target
RUB1
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.0 mg/mL
Dilution
ELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,000
Purity
Rub1 Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Rub1 Antibody is tested in western blot and defects a 6 kDa band corresponding to yeast Rub1. Most yeast cell lysates can be used as a positive control without induction or stimulation.
Tested Applications
ELISA, WB
NCBI Accession Number
Q03919.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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