RFX5 Antibody

RFX5 antibody

Product Specifications

Product Name Alternative

Rabbit anti-RFX5 antibody, DNA binding protein RFX 5 antibody, Influences HLA class II expression antibody, Regulatory factor X 5 antibody, Regulatory factor X subunit 5 antibody, Regulatory factor X 5 (influences HLA class II expression) antibody

UniProt

P48382

Reactivity

Human

Immunogen

RFX5 (N-terminal specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH) .

Target

RFX5

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

1.0 mg/mL

Dilution

ELISA: 1:5,000 - 1:25,000, Gel Supershift Assays: 0.5 to 1.0 μl, WB: 1:500 - 1:3,000

Purity

This product was prepared from monospecific antiserum by delipidation, salt fractionation and ion exchange chromatography. Anti-RFX5 (N-terminal specific) may react with unknown minor bands at 55 kDa and 85 kDa.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This product was assayed by immunoblot and found to be reactive against RFX5 (N-terminal specific) from a variety of fibroblast and B-cell lysates at a dilution of 1:500 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) . Anti-RFX5 (N-terminal specific) is suitable for the detection by immunoblot of human RFX5 a shows a 75 kDa band. This product was also tested in a gel supershift assay and found to be reactive against RFX5 complexes using 0.5 to 1.0 μl per assay.