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ALP1 Antibody

Alkaline Phosphatase antibody

Product Specifications

Product Name Alternative

Rabbit anti-Alkaline Phosphatase Antibody, rabbit anti-Alkaline phosphatase intestinal antibody, Alkaline phosphomonoesterase antibody, ALPI antibody, Glycerophosphatase antibody, IAP antibody, Intestinal alkaline phosphatase antibody, Kasahara isozyme antibody

UniProt

P09923

Reactivity

Human

Immunogen

Alkaline Phosphatase [Human Intestine]

Target

ALP1

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

1.0 mg/ml

Dilution

ELISA: 1:4,000 - 1:20,000, WB: 1:500 - 1:2,000

Purity

Anti-ALKALINE PHOSPHATASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [Human Intestine]. Cross reactivity against Alkaline Phosphatase from other sources is unknown.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

ALKALINE PHOSPHATASE Antibody has been assayed against 1.0 μg of Alkaline Phosphatase [Human Intestine] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

Tested Applications

ELISA, WB

NCBI Accession Number

NP_001622.2

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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