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TRBOTR Antibody

Trypsin antibody

Product Specifications

Product Name Alternative

Rabbit anti-trypsin antibody, Anionic trypsinogen antibody, Beta trypsin antibody, Brain trypsinogen antibody, Cationic Trypsinogen antibody, Digestive Zymogen antibody, Mesotrypsin antibody, Mesotrypsinogen antibody

UniProt

P00760

Reactivity

Bovine

Immunogen

Anti-Trypsin Antibody was produced by repeated immunizations with bovine pancreatic trypsin protein purified.

Target

TRBOTR

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

1.0 mg/mL

Dilution

ELISA: 1:5,000 - 1:20,000, WB: 1:1,000 - 1:5,000

Purity

Trypsin Antibody is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Trypsin (Bovine Pancreas) . Cross-reactivity against Trypsin from other tissues and species may occur but has not been specifically determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-Trypsin Antibody has been tested by western blotting and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Tested Applications

ELISA, WB

NCBI Accession Number

CAA38513.1

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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