NFYB Antibody

This product was prepared from monospecific antiserum by a multi-step procedure which includes delipidation, salt fractionation and ion exchange chromatography. A single precipitin arc was observed against anti-Rabbit Serum when assayed by immunoelectrophoresis.

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This product was assayed by immunoblot and found to be reactive against the 25 kDa B subunit of NF-Y at a dilution of 1:500 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code . Anti-NF-Y (B subunit specific) is suitable for the detection by immunoblot of human and mouse NF-Y (B subunit specific) . Minimal reaction was observed by immunoblot against the 35 kDa A subunit of NF-Y. This product was also tested in a gel supershift assay and found to be reactive against human and mouse NF-Y using 2.0 to 4.0 μl per assay. This product was assayed against NF-Y B subunit peptide in an antibody capture ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS substrate. A dilution of 1:4000 is suggested from this experiment. Minimal reactivity was observed by ELISA against the A subunit of NF-Y.