BrdU Antibody
BrdU antibody
Product Specifications
Product Name Alternative
Mouse anti-BrdU Antibody, Bromodeoxyuridine, 5-bromo-2'-deoxyuridine, BrdU
Reactivity
Other
Immunogen
Anti-BrdU monoclonal antibody was produced in mice by repeated immunizations prepared via immunizations with BromodeoxyUridine-KLH followed by hybridoma development.
Clonality
Monoclonal
Clone
29G6.E8
Conjugation
Unconjugated
Concentration
1.0 mg/ml
Dilution
ELISA: 1:2000 - 1:10000, FC: 1:50-1:100, IHC: 1:100-1:500, IF: 1:500-1:3000, WB: 1:2000 - 1:5000
Purity
Anti-BrdU Monoclonal Antibody was purified from ascites fluid by Protein A chromatography. This antibody reacts strongly with BrdU. Cross-reactivity is observed with CldU and IdU.
Form
Liquid (sterile filtered)
Storage Conditions
Store BrdU Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-BrdU Antibody has been tested immunofluorescence and slot blot assays. Antibody may be suitable for additional immunoassays including flow cytometry and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Antibody will detect incorporated BrdU thymidine analog from replicated cells
Tested Applications
DOT, ELISA, FC, IF, IHC, WB
Host or Source
Mouse
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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