Ido1 Antibody
IDO antibody
Product Specifications
Product Name Alternative
Mouse anti-IDO1 antibody, Ido, Indo, Indoleamine 2,3-dioxygenase 1, Indoleamine-pyrrole 2,3-dioxygenase, Ido1, Ido-1
UniProt
P14902
Reactivity
Human, Mouse
Immunogen
Anti-IDO1 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with fragment of recombinant human and mouse IDO1 protein followed by hybridoma development.
Target
Ido1
Clonality
Monoclonal
Clone
10.1
Conjugation
Unconjugated
Field of Research
Metabolism Research
Concentration
1.0 mg/mL
Dilution
ELISA: 1:5000-1:50000, IHC: 1:100-1:500, IF: 1:50-1:100, IP: 10-100 μL, WB: 1:500-1:1500
Purity
Anti-IDO1 was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human and mouse IDO1 protein. A BLAST analysis was used to suggest cross-reactivity with IDO1 from human and mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with IDO1 from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-IDO1 antibody has been tested in SDS-PAGE, ELISA, IP, and Western Blot. This antibody is suitable for use in IHC and Flow Cytometry. Specific conditions for reactivity should be optimized by the end user.
Tested Applications
ELISA, IF, IHC, IP, WB
NCBI Accession Number
NP_002155.1
Host or Source
Mouse
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG3
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