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Ido1 Antibody

IDO antibody

Product Specifications

Product Name Alternative

Mouse anti-IDO1 antibody, Ido antibody, Indo, Indoleamine 2,3-dioxygenase 1, Indoleamine-pyrrole 2,3-dioxygenase, Ido1, Ido-1

UniProt

P14902

Reactivity

Human, Mouse

Immunogen

Anti-IDO1 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with fragment of recombinant human and mouse IDO1 protein followed by hybridoma development.

Target

Ido1

Clonality

Monoclonal

Clone

10.1

Conjugation

Unconjugated

Field of Research

Metabolism Research

Concentration

1.09 mg/mL

Dilution

ELISA: 1:5000-1:50000, IHC: 1:100-1:500, IF: 1:50-1:100, IP: 10-100 μL, WB: 1:500-1:1500

Purity

Anti-IDO1 was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human and mouse IDO1 protein. A BLAST analysis was used to suggest cross-reactivity with IDO1 from human and mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with IDO1 from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-IDO1 antibody has been tested in SDS-PAGE, ELISA, IP, and Western Blot. This antibody is suitable for use in IHC and Flow Cytometry. Specific conditions for reactivity should be optimized by the end user.

Tested Applications

ELISA, IF, IHC, IP, WB

NCBI Accession Number

NP_002155.1

Host or Source

Mouse

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG3

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