LGR4 Antibody

This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human LGR4 protein. The hybridoma was produced by fusing BALB/c mouse splenocytes and mouse myeloma SP2/O cells using conventional technology.

Anti-LGR4 was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human LGR4 protein. A BLAST analysis was used to suggest cross-reactivity with LGR4 from chimpanzee, orangutan and macaque based on 100% homology with the immunizing sequence. Cross-reactivity with LGR4 from other sources has not been determined.

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Anti-LGR4 monoclonal antibody has been tested by ELISA, SDS-Page, and western blot, and is suitable in immunohistochemistry. Expect a band approximately 102 kDa in size corresponding to LGR4 protein by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. Use formalin-fixed paraffin-embedded sections for immunohistochemistry. No pre-treatment of sample is required.