LGR4 Antibody

LGR4 antibody

Product Specifications

Product Name Alternative

Mouse anti-LGR4 antibody, mouse anti-LGR 4 antibody, leucine-rich repeat-containing G protein-coupled receptor 4

UniProt

Q8N537

Reactivity

Human

Immunogen

This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human LGR4 protein. The hybridoma was produced by fusing BALB/c mouse splenocytes and mouse myeloma SP2/O cells using conventional technology.

Target

LGR4

Clonality

Monoclonal

Clone

6G8.B3.G5.C3

Conjugation

Unconjugated

Field of Research

Signal Transduction

Concentration

1.03 mg/mL

Dilution

ELISA: 1:20,000 - 1:100,000, IHC: 5 μg/mL, WB: 1:500 - 1:3,000

Purity

Anti-LGR4 purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human LGR4 protein. A BLAST analysis was used to suggest cross-reactivity with LGR4 from chimpanzee, orangutan and macaque based on 100% homology with the immunizing sequence. Cross-reactivity with LGR4 from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-LGR4 monoclonal antibody has been tested by ELISA, SDS-Page, and western blot, and is suitable in immunohistochemistry. Expect a band approximately 102 kDa in size corresponding to LGR4 protein by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. Use formalin-fixed paraffin-embedded sections for immunohistochemistry. No pre-treatment of sample is required.