Stat5a Antibody

Anti-STAT5 phospho Y694 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5a protein.

Phospho STAT5 pY694 was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for mouse Stat5a protein phosphorylated at Y694. A BLAST analysis was used to suggest cross-reactivity with Stat5a from human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Stat5a from other sources has not been determined.

Store STAT5 phospho Y694 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Phospho STAT5 antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Phospho STAT5 pY694 monoclonal antibody has been tested by ELISA, western blot, immunohistochemistry, and immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91 kDa in size corresponding to phosphorylated Stat5a protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with mouse Stat5a pY694 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.