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GAPDH Antibody

GAPDH antibody

Product Specifications

Product Name Alternative

Mouse anti-GAPDH antibody, Loading Control Antibody, G3PDH, GAPD, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH

UniProt

P46406

Reactivity

Human, Mouse, Rabbit

Immunogen

Anti-GAPDH Monoclonal Antibody was produced by repeated immunizations in mice with rabbit GAPDH protein.

Target

GAPDH

Clonality

Monoclonal

Clone

33F2.C8.G6.E3

Conjugation

Unconjugated

Field of Research

Metabolism Research

Concentration

1.0 mg/ml

Dilution

IF: 1:100, WB: 1:1,000

Purity

Anti-GAPDH Monoclonal Antibody is directed against rabbit Glyceraldehyde-3-Phosphate Dehydrogenase. The antibody is a total IgM fraction prepared from ascites fluid by selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Anti-GAPDH antibodies are expected to react with the following species based on 100% sequence homology: rabbit, human, and mouse. Reactivity against other sources is not known.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-GAPDH (Mouse) has been tested in ELISA and western blot. This product is suitable in IHC and IF. Specific conditions should be optimized by the end user. Expect a band of ~38kDa in size corresponding to Glyceraldehyde 3-Phosphate Dehydrogenase protein by Western blot in the appropriate cell lysate or extract.

Tested Applications

ELISA, IF, Multiplex Assay, WB

NCBI Accession Number

NP_001075722.1

Host or Source

Mouse

Preservative

Preservative: 0.1% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0

Isotype

IgM

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