GAPDH Antibody
GAPDH antibody
Product Specifications
Product Name Alternative
Mouse anti-GAPDH antibody, Loading Control Antibody, G3PDH, GAPD, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH
UniProt
P46406
Reactivity
Human, Mouse, Rabbit
Immunogen
Anti-GAPDH Monoclonal Antibody was produced by repeated immunizations in mice with rabbit GAPDH protein.
Target
GAPDH
Clonality
Monoclonal
Clone
33F2.C8.G6.E3
Conjugation
Unconjugated
Field of Research
Metabolism Research
Concentration
1.0 mg/ml
Dilution
IF: 1:100, WB: 1:1,000
Purity
Anti-GAPDH Monoclonal Antibody is directed against rabbit Glyceraldehyde-3-Phosphate Dehydrogenase. The antibody is a total IgM fraction prepared from ascites fluid by selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Anti-GAPDH antibodies are expected to react with the following species based on 100% sequence homology: rabbit, human, and mouse. Reactivity against other sources is not known.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-GAPDH (Mouse) has been tested in ELISA and western blot. This product is suitable in IHC and IF. Specific conditions should be optimized by the end user. Expect a band of ~38kDa in size corresponding to Glyceraldehyde 3-Phosphate Dehydrogenase protein by Western blot in the appropriate cell lysate or extract.
Tested Applications
ELISA, IF, Multiplex Assay, WB
NCBI Accession Number
NP_001075722.1
Host or Source
Mouse
Preservative
Preservative: 0.1% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Isotype
IgM
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