Fluorescein (FITC) Monoclonal Antibody
Fluorescein antibody
Product Specifications
Product Name Alternative
Mouse Anti-FITC antibody, mouse anti-fluorescein antibody
Immunogen
Fluorescein conjugated to Mouse IgG
Clonality
Monoclonal
Clone
8B9.C6.D3
Conjugation
Unconjugated
Concentration
1.0 mg/ml
Dilution
ELISA: 1:5,000 - 1:30,000, FC: 1-2 μg/mL, IHC: 1:250 - 1:1,500, WB: 1:500 - 1:3,000
Purity
Fluorescein Antibody was prepared from mouse ascites by Protein A chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-mouse serum, Fluorescein conjugated IgG and Fluorescein conjugated Bovine Serum Albumin.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Fluorescein Antibody has been tested by ELISA, western blot, and immunofluorescence. This product is suitable for immunomicroscopy, in situ hybridization and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Tested Applications
ELISA, FC, IF, IHC, WB
Host or Source
Mouse
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG2a
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