Zwf Antibody (Biotin)

Goat anti-Glucose-6-Phosphate Dehydrogenase Antibody biotin Conjugation, biotin Conjugated goat anti-Glucose-6-Phosphate Dehydrogenase Antibody, G6PD antibody, G6PD1 antibody, G6pdx antibody, Glucose 6 phosphate 1 dehydrogenase antibody, MET19 antibody, POS10 antibody, Zwf1p antibody

Anti-Glucose-6-Phosphate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Glucose-6-Phosphate Dehydrogenase [Leuconostoc mesenteroides]. Cross reactivity against Glucose-6-Phosphate Dehydrogenase from other sources may occur but have not been specifically determined.

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Anti-Glucose-6-Phosphate Dehydrogenase Biotin has been tested by ELISA and western blot. This product is assayed against 1.0 ug of Glucose-6-Phosphate Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2