PGA Antibody
Pepsin antibody
Product Specifications
Product Name Alternative
Goat anti-Pepsin Antibody, Pepsin A antibody, Pepsin a precursor antibody, Pepsin antibody, Pepsinogen 1 second locus antibody, Pepsinogen 3 group 1 antibody, Pepsinogen a3 antibody
UniProt
P00791
Reactivity
Porcine
Immunogen
Anti-Pepsin Antibody was produced by repeated immunizations with pork Pepsin protein isolated from swine stomach.
Target
PGA
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.0 mg/ml
Dilution
ELISA: 1:50:000 - 1:200,000, IHC: 1:2,000 - 1:10,000, IP: 1:100, WB: 1:5,000 - 1:20,000
Purity
Anti-PEPSIN is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Pepsin [Porcine Stomach]. Cross reactivity against Pepsin from other tissues and species may occur but have not been specifically determined.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Pepsin antibody has been tested in ELISA, western blot and immunohistochemistry. This product detects a single band of the expected apparent molecular weight and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
Tested Applications
ELISA, IHC, IP, WB
NCBI Accession Number
AAA31095.1
Host or Source
Goat
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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