PPI Antibody
Papain antibody
Product Specifications
Product Name Alternative
Goat anti-Papain Antibody, Allergen Car p 1 antibody, Papaya proteinase I antibody, PPI antibody
UniProt
P00784
Reactivity
Plant
Immunogen
Anti-Papain Antibody was produced by repeated immunizations with pork Papain protein isolated from papaya.
Target
PPI
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.0 mg/ml
Dilution
ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:3,000, WB: 1:1,000 - 1:5,000
Purity
Anti-Papain antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Papain [Carica papaya]. Cross reactivity against Papain from other sources may occur but has not been specifically determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-Papain antibody has been tested by western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
P00784.1
Host or Source
Goat
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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