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CA1 Antibody

Carbonic Anhydrase I antibody

Product Specifications

Product Name Alternative

Goat anti-Carbonic Anhydrase I Antibody, Car1 antibody, Carbonate dehydratase I antibody, Carbonic anhydrase 1 antibody, Carbonic anhydrase B antibody, Carbonic dehydratase antibody, ECK0125 antibody, yadF antibody

UniProt

P00915

Reactivity

Human

Immunogen

Carbonic Anhydrase I [Human Erythrocytes]

Target

CA1

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

1.0 mg/ml

Dilution

ELISA: 1:2,000 - 1:20,000, IHC: 10μg/ml, WB: 1:500 - 1:2,000

Purity

Anti-CARBONIC ANHYDRASE I is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Carbonic Anhydrase I [Human Erythrocytes]. Cross reactivity against Carbonic Anhydrase I from other tissues and species may occur but have not been specifically determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-Carbonic Anhydrase 1 has been tested in western blot and immunohistochemistry and is suitable in ELISA. Specific conditions for reactivity should be optimized by the end user.

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

NP_001122301.1

Host or Source

Goat

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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