Acetylcholinesterase Antibody Peroxidase Conjugated

Goat anti-Acetyl Cholinesterase Antibody, HRP conjugated goat anti-Acetyl Cholinesterase Antibody, Acetylcholinesterase, AChE, HRP, Horseradish peroxidase, HRP, Horseradish peroxidase

Anti-Acetylcholinesterase (Bovine Erythrocytes) antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Acetylcholinesterase [Bovine Erythrocytes]. Cross reactivity against Acetylcholinesterase from other sources is unknown.

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Anti-Acetylcholinesterase (Bovine Erythrocytes) antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Acetylcholinesterase [Bovine Erythrocytes] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 of the reconstitution concentration is suggested for this product.

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2