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GdhB Antibody (HRP)

Glucose Dehydrogenase antibody (Peroxidase)

Product Specifications

Product Name Alternative

Goat anti-Glucose Dehydrogenase Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Glucose Dehydrogenase Antibody, gdh antibody, gdh2 antibody, gdh3 antibody, gdh4 antibody, gdhI antibody, GLCDHIII antibody, GLCDHIV antibody, Glucose 1 dehydrogenase 1 antibody

UniProt

P07999

Reactivity

Bacteria

Immunogen

Glucose Dehydrogenase [Bacillus]

Target

GdhB

Clonality

Polyclonal

Conjugation

HRP

Concentration

1 mg/mL

Dilution

ELISA: 1:10,000 - 1:40,000, WB: 1:1,000 - 1:5,000

Purity

Glucose Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Glucose Dehydrogenase [Bacillus]. Cross reactivity against Glucose Dehydrogenase from other sources is unknown.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Glucose Dehydrogenase has been assayed against 1.0 μg of Glucose Dehydrogenase [Bacillus] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product.

Tested Applications

ELISA, WB

NCBI Accession Number

AAA22475.1

Host or Source

Goat

Preservative

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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