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A2M Antibody (HRP)

Alpha-2-Macroglobulin antibody (Peroxidase)

Product Specifications

Product Name Alternative

Goat anti-Alpha-2-Macroglobulin Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Alpha-2-Macroglobulin Antibody, A2M antibody, Alpha 2 M antibody, Alpha 2M antibody, CPAMD5 antibody, DKFZp779B086 antibody, FWP007 antibody, S863 7 antibody

UniProt

P01023

Reactivity

Human

Immunogen

A2-Macroglobulin [Human Plasma]

Target

A2M

Clonality

Polyclonal

Conjugation

HRP

Concentration

1.0 mg/ml

Dilution

ELISA: 1:400 - 1:2,000, IHC: 1:50 - 1:250, WB: 1:100 - 1:500

Purity

Anti-Alpha 2-Macroglobulin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified a2-Macroglobulin [Human Plasma]. Cross reactivity against a2-Macroglobulin from other sources is unknown.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Alpha 2-Macroglobulin has been tested by dot blot and western blot and is suitable to be assayed against 1.0 ug of alpha-Macroglobulin [Human Plasma] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:12,000 to 1:60,000 of the reconstitution concentration is suggested for this product.

Tested Applications

DOT, ELISA, IHC, WB

NCBI Accession Number

NP_000005.2

Host or Source

Goat

Preservative

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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