FOLR1 Antibody

Goat anti-Folate Binding Protein Antibody, Folate receptor alpha, FR-alpha, Folate receptor 1, Milk folate-binding protein, Folate-binding protein 1, FBP

Anti-FOLATE BINDING PROTEIN is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Folate Binding Protein [Bovine Milk]. Cross reactivity against Folate Binding Protein from other tissues and species may occur but have not been specifically determined.

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

FOLATE BINDING PROTEIN Antibody has been assayed against 1.0 ug of Folate Binding Protein [Bovine Milk] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product. Highest levels of antigen binding and antibody reactivity were observed using NuncÔ brand MaxiSorp immunoplates.