Mouse anti Human CD10 FITC - CD19 PE

Anti-hCD10 recognizes a Human common acute lymphoblastic antigen (CALLA), MW 100 kDa. The CD10 antigen is identical to Human membrane-associated neutral endopeptidase (NEP;EC 3.3.24.11), also known as enkephalinase. The CD10 antigen is found on lymphocytes from patients with acute B-lymphoid leukemia. The antigen is also present on a wide variety of normal and neoplastic cell types including renal epithellum, fibroblasts, granulocytes and some lymphoma, melanoma and glioma cell lines. Identification of CD19 PE Human B cells associated approximately 10% of peripheral blood lymphocytes expressing the 95,000 M.W. surface antigen.

PBMC: Add 10 µL of MAB/10^6 PBMC in 100 µL PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze. WHOLE BLOOD: Add10 µL of MAB/100 µL of whole blood. Mix gently and incubate for 15 minutes at room temperature 20ºC. Lyse the whole blood. Wash once with PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

1. CD10/Neutral Endopeptidase 24.11 Hydrolyzes Bombesin-like Peptides and Regulates the Growth of Small Cell Carcinomas of the Lung. Proc Natl Acad Sci USA 1991, Dec1;88(23):10662-6. Shipp, MA, Tarr, GE; Chen, CY; Switzer, SN; Hersh, LB; Stein,H; Sunday, ME; Reinherz, EL. 2 Induction of Isotype Switching and Ig Production by CD5+ and CD10+ Human Fetal B Cells. JI, 1992, Jun1;148(11):3398-404. Punnonen, J; Aversa, GG;Vandekerckhove, B; Roncarolo, MG; de Vries, JE. 3. Murine Common Acute Lymphoblastic Leukemia Antigen (CD10 Neutral Endopeptidase 24.11). Molecular Characterization, Chromosomal Localization and Modeling of the Active Site. JI, 1992, May1;148(9):2817-25. Chen, CY; Salles, G; Seldin, MF; Kister, AE; Reinherz, EL; Shipp, MA. 4. Childhood Acute Leukemia with t(11;19) (q23;p13). SO. Leukemia, 1991, Dec;5(12):1064-8. Hudson, MM; Raimondi, SC; Behm, FG; Pui, CH. 5. CD10 (CALLA)/Neutral Endopeptidase 24.11 Modulates Inflammatory Peptide-Induced Changes in Neutrophil Morphology, Migration and Adhesion Proteins and is itself Regulated by Neutrophil Activation. Blood, 1991, Oct. 1;78(7):1834-41. Shipp, MA; Stefano, GB; Switzer, SN; Griffin, JD; Reinherz, EL. 6. Functional Properties of CD19+ B Lymphocytes Positively Selected from Buffy Coats by Immunomagnetic Separation. Funderud, S., Erikstien, B., Asheim, H.C., Nustad ,K., Stokke, T., Blomhoff, H.K., Holte, H., Smeland, E.B.. Eur. J. Immunol. 1990 Ja;20(1):2016 7. Thymic B Cells from Myasthenia Gravis Patients are Activated B Cells. Phenotypic and Functional Analysis. Leprince, C., CohenKaminsky, S., Berrih-Aknin, S., Vernet-Der Garabedian, B., Treton, D., Galanaud, P., Richard, Y., J. Immunol. 1990 Oct., 145(7):2115-22. 8. Prognostic Significance of CD34 Expression in Childhood B Precursor Acute Lymphocytic Leukemia: A Pediatric Onocology Group Study. Borowitz, M.J., Shuster, J.J., Civin, C.I., Carrol, A.J., Look, A.T., Behm, F.G., Land, V.J., Pullen, D.J., Crist, W.M.. J. Clin. Onol. 1990 Au;8(8):1389-98. 9. Biphenotypic Acute Leukemia in Adults. Sulak, L.E., Clare, C.N., Morale, B.A., Hansen, K.L., Montiel, M.M.. Am. J. Clin. Path. 1990 Ju;94(1):54-8. 10. Intersection of the Complement and Immune Systems: A Signal Transduction Complex of the B Lymphocyte Containing Complement Receptor type 2 and CD19. Matsumoto, A.K., Kopicky-Burd , J., Carter, R.H., Tuveson, D.A., Tedder, T.F., Fearon, D.T., J. Exp. Med. 1991 Jan. 173(1):55-64.