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Human IgG Biotin Antibody

Human IgG Biotin Antibody

Product Specifications

Product Name Alternative

Human IgG Biotin conjugation, Human immunoglobulin G, Vitamin H, coenzyme R

Conjugation

Biotin

Source

Human

Field of Research

Protein Biochemistry

Concentration

1.040 mg/mL

Dilution

ELISA: 1:5000 - 1:50,000, IHC: 1:500, WB: 1:1000

Purity

Human IgG whole molecule Biotin conjugated was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by extensive dialysis against the buffer stated above. Human IgG whole molecule Biotin conjugated was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Human IgG and anti-Human Serum.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Human IgG whole molecule Biotin conjugated is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Human IgG whole molecule Biotin conjugated has been tested in ELISA and can be utilized as a control reagent in both Western Blotting and ELISA format experiments.

Tested Applications

ELISA, IHC, WB

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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