Human IgG Biotin Antibody
Human IgG Biotin Antibody
Product Specifications
Product Name Alternative
Human IgG Biotin conjugation, Human immunoglobulin G, Vitamin H, coenzyme R
Conjugation
Biotin
Source
Human
Field of Research
Protein Biochemistry
Concentration
1.040 mg/mL
Dilution
ELISA: 1:5000 - 1:50,000, IHC: 1:500, WB: 1:1000
Purity
Human IgG whole molecule Biotin conjugated was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by extensive dialysis against the buffer stated above. Human IgG whole molecule Biotin conjugated was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Human IgG and anti-Human Serum.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Human IgG whole molecule Biotin conjugated is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Human IgG whole molecule Biotin conjugated has been tested in ELISA and can be utilized as a control reagent in both Western Blotting and ELISA format experiments.
Tested Applications
ELISA, IHC, WB
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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