C6 NBD Ceramide

C6 NBD Ceramide is a Golgi apparatus fluorescent probe with cell membrane permeability. C6 NBD Ceramide can be used for fast and convenient green fluorescent labeling of Golgi in living and fixed cells, and can be used to observe changes in Golgi morphology in living cells (Ex=466 nm, Em=536 nm) . C6-NBD-ceramide is metabolized to fluorescent sphingomyelin and glucosylceramide, can be used for the study of sphingolipid transport and metabolic mechanism[1][2][3].

Product Specifications

CAS Number

[94885-02-6]

Product Name Alternative

Nbd-ceramide

UNSPSC

12171500

Hazard Statement

H302, H315, H319, H335

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/c6-nbd-ceramide.html

Purity

99.33

Solubility

10 mM in DMSO

Smiles

CCCCCCCCCCCCC/C=C/[C@@H](O)[C@@H](NC(CCCCCNC1=CC=C([N+]([O-])=O)C2=NON=C12)=O)CO

Molecular Formula

C30H49N5O6

Molecular Weight

575.74

Precautions

H302, H315, H319, H335

References & Citations

[1]Lipsky NG, et al. A vital stain for the Golgi apparatus. Science. 1985 May 10;228 (4700) :745-7. |[2]Brandán YR, et al. Influence of sphingomyelin metabolism during epithelial-mesenchymal transition associated with aging in the renal papilla. J Cell Physiol. 2022 Jul 31. |[3]Pagano RE. A fluorescent derivative of ceramide: physical properties and use in studying the Golgi apparatus of animal cells. Methods Cell Biol. 1989;29:75-85.