Anti-CD46/Membrane Cofactor Protein Antibody

Reconstitute lyophilized antibody using ultrapure to a final concentration no less than 100 ug/mL. Reconstitution with PBS or Tris buffer is acceptable if required. Protein carrier, biocide or cryopreservative can be added as needed. Aliquot and store at -20C for longterm storage. Dilute immediately prior to use.

Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. {PubMed:10843656, PubMed:12540904}.; (Microbial infection) A number of viral and bacterial pathogens seem to bind MCP in order to exploit its immune regulation property and directly induce an immunosuppressive phenotype in T-cells.; (Microbial infection) Acts as a receptor for Adenovirus subgroup B2 and Ad3. {PubMed:12915534, PubMed:14566335, PubMed:15047806, PubMed:15078926, PubMed:15919905, PubMed:16254377}.; (Microbial infection) Acts as a receptor for cultured Measles virus. {PubMed:10972291}.; (Microbial infection) Acts as a receptor for Herpesvirus 6/HHV-6. {PubMed:12663806, PubMed:12724329}.; (Microbial infection) May act as a receptor for pathogenic bacteria Neisseria and Streptococcus pyogenes (PubMed:7708671, PubMed:9379894, PubMed:11260136, PubMed:11971006).

<br><br>Immunohistochemistry: use at 1- 10ug/ml. <br><br>Immunoblotting: use at 0.5-1ug/ml. A band of ~44kDa is detected. Additional bands due to glycosylation of CD46 may be detected.ELISA: use at 0.1-1ug/ml with human CD46 on the solid phase. <br><br>These are recommended concentrations. <br><br>Enduser should determine optimal concentrations for their applications.

This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles.

CD46 / Membrane Cofactor Protein is a type I membrane protein and is a regulatory part of the complement system; it has cofactor activity for inactivation of complement components C3b and C4b by serum factor I which protects the host cell from damage by complement. This protein also acts as a receptor for the Edmonston strain of measles virus, human Herpes virus-6 (HHV-6), and type IV pili of pathogenic Neisseria. Of interest is that medulloblastoma (a malignant brain tumor common in childhood) specimens express a high level of CD46.