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Biotinylated Mouse CD200 / OX-2 Protein, Fc, Avitag™ (MALS verified)

CD200 is also known as OX-2 membrane glycoprotein (OX-2), is a type-1 membrane glycoprotein, which contains two immunoglobulin domains (1 Ig-like C2-type (immunoglobulin-like) domain and Ig-like V-type (immunoglobulin-like) domain), and thus belongs to the immunoglobulin superfamily. CD200 / OX-2 is widely expressed in multiple cell types. CD200 interacts with a structurally related receptor (CD200R) expressed mainly on myeloid cells and is involved in regulation of macrophage and mast cell function. OX-2 / CD200 and CD200R associate via their respective N-terminal Ig-like domains. CD200 also plays an important role in prevention of graft rejection, autoimmune diseases and spontaneous abortion.

Product Specifications

Background

CD200 is also known as OX-2 membrane glycoprotein (OX-2), is a type-1 membrane glycoprotein, which contains two immunoglobulin domains (1 Ig-like C2-type (immunoglobulin-like) domain and Ig-like V-type (immunoglobulin-like) domain), and thus belongs to the immunoglobulin superfamily. CD200 / OX-2 is widely expressed in multiple cell types. CD200 interacts with a structurally related receptor (CD200R) expressed mainly on myeloid cells and is involved in regulation of macrophage and mast cell function. OX-2 / CD200 and CD200R associate via their respective N-terminal Ig-like domains. CD200 also plays an important role in prevention of graft rejection, autoimmune diseases and spontaneous abortion.

Specifications

This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 50.5 kDa. The protein migrates as 66-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

O54901-1

Expression Region

Gln 31 - Gly 232

Host

HEK293

Target

CD200

Conjugation

Biotin-labeled

Tag

C-Fc & C-Avi

Source

Mouse

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human CD200 R1, His Tag (Cat. No. CR2-H52H6) at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse CD200, Fc,Avitag (Cat. No. OX2-M82F3) with a linear range of 0.02-0.5 μg/mL (QC tested).

Format

Powder

Buffer

50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5

Additives

Trehalose

Molecular Weight

50.5 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

200ug*1

Host or Source

HEK293

Species

Mouse

Protein ID

O54901-1

Preservative

Trehalose

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