Human DNAM-1 / CD226 Protein, Fc Tag (MALS verified)
Human DNAM-1, Fc Tag (DN1-H5257) is expressed from human 293 cells (HEK293) . It contains AA Glu 19 - Asn 247 (Accession # NP_006557) .
Product Specifications
Background
DNAX accessory molecule 1 (DNAM-1), a single-pass type I membrane protein, is also known as CD226 antigen and platelet and T cell activation antigen 1 (PTA1), which contains 2 Ig-like C2-type (immunoglobulin-like) domains. DNAM-1 is a ~65 kDa glycoprotein expressed on the surface of natural killer cells, platelets, monocytes and a subset of T cells. DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.
Specifications
This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 52.6 kDa. The protein migrates as 65-85 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
NP_006557
Expression Region
Glu 19 - Asn 247
Host
HEK293
Target
DNAM-1
Conjugation
Unconjugated
Tag
C-Fc
Source
Human
Stability
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
95%
Bioactivity
Immobilized Rhesus macaque CD155 (Cat. No. CD5-R52H5) at 5 μg/mL (100 μL/well) can bind Human DNAM-1, Fc Tag (Cat. No. DN1-H5257) with a linear range of 2-31 ng/mL (QC tested).
Format
Powder
Buffer
Tris with Glycine, Arginine and NaCl, pH7.5
Additives
Trehalose
Molecular Weight
52.6 kDa
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
100ug*1
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/DN1-H5257.pdf
Species
Human
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
