3,4-Dichloroisocoumarin

3,4-Dichloroisocoumarin is a potent serine-protease and SrLip inhibitor (Ki for SrLip: 26.6 μM) . 3,4-Dichloroisocoumarin is opened by serine proteases and then undergoes acylation with the enzyme, thereby inhibiting protease activity. 3,4-Dichloroisocoumarin can induce DNA fragmentation and Apoptosis. 3,4-Dichloroisocoumarin can be used in the research of multiple fields such as tumors, cardiovascular disease and enzyme catalytic mechanisms[1][2][3][4][5].

Product Specifications

CAS Number

[51050-59-0]

UNSPSC

12352005

Hazard Statement

H301, H315, H319, H335

Target

Apoptosis; Ser/Thr Protease

Type

Reference compound

Related Pathways

Apoptosis; Metabolic Enzyme/Protease

Applications

Metabolism-protein/nucleotide metabolism

Field of Research

Cancer; Metabolic Disease; Cardiovascular Disease

Assay Protocol

https://www.medchemexpress.com/3-4-dichloroisocoumarin.html

Purity

99.72

Solubility

DMSO : 100 mg/mL (ultrasonic)

Smiles

ClC1=C(OC(C2=C1C=CC=C2)=O)Cl

Molecular Formula

C9H4Cl2O2

Molecular Weight

215.03

Precautions

H301, H315, H319, H335

References & Citations

[1]Hameed A, et al. 3,4-Dichloroisocoumarin serine protease inhibitor induces DNA fragmentation and apoptosis in susceptible target cells. Proc Soc Exp Biol Med. 1998 Nov;219 (2) :132-7.|[2]Anees M, et al. Inhibition of a tumour protease with 3,4-dichloroisocoumarin, pentamidine-isethionate and guanidino derivatives. J Enzyme Inhib. 1994;8 (3) :213-21.|[3]Pereira ME, et al. 3,4-dichloroisocoumarin-induced activation of the degradation of beta-casein by the bovine pituitary multicatalytic proteinase complex. J Biol Chem. 1992 Apr 15;267 (11) :7949-55.|[4]Ašler IL, et al. Inhibition of extracellular lipase from Streptomyces rimosus with 3,4-dichloroisocoumarin. J Enzyme Inhib Med Chem. 2013 Oct;28 (5) :1094-104.|[5]Minatoguchi S, et al. Caspase-dependent and serine protease-dependent DNA fragmentation of myocytes in the ischemia-reperfused rabbit heart: these inhibitors do not reduce infarct size. Jpn Circ J. 2001 Oct;65 (10) :907-11.