Calprotectin (Human) ELISA Kit

Calprotectin is a calcium and zinc-binding protein that belongs to the family of S100 proteins. It is a 24 kDa dimer that is formed by monomers S100A8 and S100A9. It is predominantly present in neutrophils but less in monocytes and macrophages. Calprotectin contributes to approximately 60% of the cytosolic proteins in neutrophils. It has bactericidal and fungicidal properties and increases 5-40 folds during infection and inflammation. The concentration of Calprotectin in stool samples is 6 times higher than the blood plasma. Therefore it serves as a reliable non-invasive marker for inflammatory bowel diseases such as ulcerative colitis and Crohn's disease. BioVision’s Calprotectin (Human) ELISA kit is based on the Sandwich ELISA principle to quantitatively measure the amount of Calprotectin in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of Calprotectin in the sample or standard.