Anti-Aha1 Antibody

Mouse Monoclonal Antibody specific to Aha1

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

AHA1, p38

Gene Name

AHSA1

Gene ID

10598

NCBI Gene ID

UniProt

O95433

Accession Number

NP_036243.1

Cellular Locus

Cytoplasm, cytosol, Endoplasmic reticulum

Host

Mouse

Reactivity

Human, Mouse

Immunogen

His-tagged recombinant human Aha1 (accession no. NP_036243.1)

Target Antigen

Activator of 90 kDa heat shock protein ATPase homolog 1

Target

Aha1

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, ELISA

Field of Research

Heat Shock& Stress Proteins

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Acts as a co-chaperone of HSP90AA1 (PubMed:29127155). Activates the ATPase activity of HSP90AA1 leading to increase in its chaperone activity (PubMed:29127155). Competes with the inhibitory co-chaperone FNIP1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (PubMed:27353360). Competes with the inhibitory co-chaperone TSC1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (PubMed:29127155). {PubMed:27353360, PubMed:29127155}.

Additionnal Information

Immunoblotting: use at 1ug/mL. A band of ~38kDa is detected. Band can appear as high as 45kDa ELISA: use at 1-10ug/mL with Aha1 on the solid phase. These are recommended concentrations; Enduser should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human and mouse Aha1 and cross-reacts with mouse Aha2.

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography.

Buffer pH

pH 7.4

Target Background

Aha1 is a member of the Hsp90 co- chaperone family that appears to stimulate Hsp90 ATPase activity by competing with p23 and other co- chaperones for Hsp90 binding. Aha1 also interacts with HSPCA/Hsp90 and with the cytoplasmic tail of the vesicular stomatitis virus glycoproteins (VSV-G). Aha 1 is expressed in numerous tissues including brain, heart, skeletal muscle, and kidney and is present at low levels in liver and placenta.