Anti-Ataxin-1 Antibody

Mouse Monoclonal Antibody specific to Ataxin-1

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Spinocerebellar ataxia type 1 protein homolog

Gene Name

Atxn1

NCBI Gene ID

UniProt

P54254

Cellular Locus

Cytoplasm, Nucleus

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to aa 164-197 (ATTPSQRSQLEAYSTLLAN- MGSLSQAPGHKVEPP) of mouse Ataxin-1. This sequence is 100

Target Antigen

Ataxin-1

Target

Ataxin-1

Clonality

Monoclonal

Isotype

IgG2b

Type

Antibody

Applications

WB, IHC, ICC/IF, IP

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Chromatin-binding factor that repress Notch signaling in the absence of Notch intracellular domain by acting as a CBF1 corepressor. Binds to the HEY promoter and might assist, along with NCOR2, RBPJ-mediated repression (By similarity). May be involved in RNA metabolism (By similarity). In concert with CIC and ATXN1L, involved in brain development (PubMed:28288114). {UniProtKB:P54253, PubMed:28288114}.

Additionnal Information

Immunoblotting: use at 1-5ug/mL. A band of ~85kDa is detected. Immunofluorescence: use at 10ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their application.

Storage Conditions

This product is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse, and rat Ataxin-1.

Formulation

PBS, pH 7.4, 0.1% sodium azide, 50% glycerol.

Buffer pH

pH 7.4

Target Background

Ataxin-1 is a member of the ATXN1 protein family. It is a neurodegenerative disorder protein that is believed to play a role in metabolism of RNA. A mutation of Ataxin-1 causes spinocerebellar ataxia type-1 (SCA1), a progressive neurodegenerative disease that primarily affects Purjinke cells in the brain stem and cerebellum. Ataxin-1 is ubiquitously expressed except in brain where it is limited to populations of neurons.