Ascorbate Oxidase

<strong>Ascorbate Oxidase (Microbial).</strong>_x000D_ <table style="height: 581px;" width="452">_x000D_ <tbody>_x000D_ <tr>_x000D_ <td><strong>Catalog #</strong></td>_x000D_ <td>P2010007</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td><strong>Size</strong></td>_x000D_ <td>1000 U</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td><strong>Unit Definition</strong></td>_x000D_ <td>Amount of enzyme needed to oxidize 1 μmole of ascorbate to dehydroascorbate per minute at 30 ℃.</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td><strong>Molecular Weight</strong></td>_x000D_ <td>80 kDa</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td><strong>Specific Activity</strong></td>_x000D_ <td>350 U/mg</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td><strong>Purity</strong></td>_x000D_ <td>Uricase, phosphatase &lt; 0.001%</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td><strong>Storage</strong></td>_x000D_ <td>-20°C. Avoid repeated freeze/thaw cycles.</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td><strong>Supplied as</strong></td>_x000D_ <td>Light Blue lyophilized powder</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td><strong>Keywords</strong></td>_x000D_ <td>Ascorbate oxidase, L-ascorbic acid oxidase, ascorbic acid</td>_x000D_ </tr>_x000D_ </tbody>_x000D_ </table>_x000D_ &nbsp;_x000D_ _x000D_ <strong>References</strong>_x000D_ <ul>_x000D_ <li>A novel case of ACOX2 deficiency leads to recognition of a third human peroxisomal acyl-CoA oxidase. <span class="labs-docsum-authors full-authors">Ferdinandusse S, Denis S, van Roermund CWT, Preece MA, Koster J, Ebberink MS, Waterham HR, Wanders RJA.</span> <span class="labs-docsum-journal-citation full-journal-citation">Biochim Biophys Acta Mol Basis Dis. 2018 Mar;1864(3):952-958.</span></li>_x000D_ <li>Oxidase Activity of the Barnacle Adhesive Interface Involves Peroxide-Dependent Catechol Oxidase and Lysyl Oxidase Enzymes.<span class="labs-docsum-authors full-authors"> So CR, Scancella JM, Fears KP, Essock-Burns T, Haynes SE, Leary DH, Diana Z, Wang C, North S, Oh CS, Wang Z, Orihuela B, Rittschof D, Spillmann CM, Wahl KJ.</span> <span class="labs-docsum-journal-citation full-journal-citation">ACS Appl Mater Interfaces. 2017 Apr 5;9(13):11493-11505.</span></li>_x000D_ <li>Zymographic Determination of Intrinsic Specific Activity of Oxidases in the Presence of Interfering Proteins.<span class="labs-docsum-authors full-authors"> Le TC, Mateescu MA, Ahmadifar S, Marcocci L, Pietrangeli P.</span> <span class="labs-docsum-journal-citation full-journal-citation">Methods Mol Biol. 2018;1853:207-221.</span></li>_x000D_ <li>A biochemical approach to study the role of the terminal oxidases in aerobic respiration in Shewanella oneidensis MR-1.<span class="labs-docsum-authors full-authors"> Le Laz S, Kpebe A, Bauzan M, Lignon S, Rousset M, Brugna M.</span> <span class="labs-docsum-journal-citation full-journal-citation">PLoS One. 2014 Jan 22;9(1):e86343. </span></li>_x000D_ <li>The crystal structure of an extracellular catechol oxidase from the ascomycete fungus Aspergillus oryzae.<span class="labs-docsum-authors full-authors"> Hakulinen N, Gasparetti C, Kaljunen H, Kruus K, Rouvinen J.</span> <span class="labs-docsum-journal-citation full-journal-citation">J Biol Inorg Chem. 2013 Dec;18(8):917-29.</span></li>_x000D_ <li>Immunoblot analyses of the elicited Sanguinaria canadensis enzyme, dihydrobenzophenanthridine oxidase: evidence for resolution from a polyphenol oxidase isozyme.<span class="labs-docsum-authors full-authors"> Ignatov A, Neuman MC, Barg R, Krueger RJ, Coscia CJ.</span> <span class="labs-docsum-journal-citation full-journal-citation">Arch Biochem Biophys. 1997 Nov 15;347(2):208-12. </span></li>_x000D_ <li>Defining the role of tyrosine and rational tuning of oxidase activity by genetic incorporation of unnatural tyrosine analogs.<span class="labs-docsum-authors full-authors"> Yu Y, Lv X, Li J, Zhou Q, Cui C, Hosseinzadeh P, Mukherjee A, Nilges MJ, Wang J, Lu Y.</span> <span class="labs-docsum-journal-citation full-journal-citation"><a href="https://pubmed.ncbi.nlm.nih.gov/25672571/">J Am Chem Soc.</a> 2015 Apr 15;137(14):4594-7. </span></li>_x000D_ </ul>