SARS-CoV-2 Nucleocapsid protein (R203K, G204R), His Tag

SARS-CoV-2 Nucleocapsid protein (R203K, G204R), His Tag (NUN-C52Hg) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # QHO62115.1 (R203K, G204R)). The nucleocapsid protein is consisted of the N-terminal RNA-binding domain (NTD) and the C-terminal dimerization domain (CTD), divided by a central serine/arginine-rich (SR)-linker responsible for phosphorylation. The mutations (R203K, G204R) were identified on the SR-linker on the nucleocapsid protein of SARS-CoV-2 variants. Phosphorylation of this SR-link motif in SARS-CoV modulates nucleocapsid multimerization, translational inhibitory activity and cellular localization. The co-occuring mutations R203K and G204R may decrease the overall structural flexibility of SARS-COV-2 N protein.