SARS-CoV-2 (COVID-19) S2 protein, His Tag

SARS-CoV-2 S2 protein, His Tag (S2N-C52H2) is expressed from human 293 cells (HEK293) .

Product Specifications

Background

It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 60.0 kDa. The protein migrates as 65-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

QHD43416.1

Expression Region

Ser 686 - Pro1213

Host

HEK293

Target

Spike S2

Conjugation

Unconjugated

Tag

C-10xHis

Source

SARS-CoV-2

Stability

The product MUST be stored at -70°C or lower upon receipt; -70°C for 3 months under sterile conditions.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized SARS-CoV-2 S2 protein, His Tag (Cat. No. S2N-C52H2) at 0.2 μg/mL (100 ug/well) can bind Anti-SARS-CoV-2 Spike S2 protein Antibody, Human IgG4 (AS86) (Cat. No. S2N-S86) with a linear range of 1.5-2.5 ng/mL (QC tested).