Anti-HA Antibody
Rabbit Polyclonal Antibody specific to HA
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Anti-HA Rabbit Polyclonal Antibody
Host
Rabbit
Reactivity
Ha
Immunogen
HA (YPYDVPDYA) conjugated to KLH.
Target
HA
Clonality
Polyclonal
Isotype
Whole IgG
Type
Antibody
Applications
WB, IP, ICC, ELISA
Field of Research
Epitope Tags
Purification Method
Purified by peptide immuno-affinity chromatography
Concentration
1.0 mg/mL
Dilution
Dilute in PBS or medium that is identical to that used in the assay system.
Format
Purified
Form
Liquid
Buffer
Phosphate Buffered Saline
Additionnal Information
Immunoblotting: use at a dilution of 1:1,000- 1:20,000.
<br><br>Immunocytochemistry: use at a dilution of 1:100-1:400.
<br><br>Immunoprecipitation: use 1-4ug/mg lysate.
<br><br>ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100-1:500
<br><br>These are recommended dilutions.
<br><br>Endusers should determine optimal dilutions for their applications.
Storage Conditions
This antibody is stable for at least one (1) year at 2-8°C.
Specificity
Antibody was affinity-purified on the peptide.
Formulation
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Buffer pH
pH 7.2
Target Background
The HA epitope tag is derived from the HA-molecule corresponding to amino acids YPYDVPDYA of human influenza hemagglutinin. It is frequently used as an epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein.
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