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F (ab') 2 Chicken IgG (H&L) Antibody Peroxidase Conjugated

F (ab') 2 Chicken IgG (H&L) antibody (Peroxidase)

Product Specifications

Product Name Alternative

Rabbit F (ab') 2 anti-Chicken IgG Antibody peroxidase Conjugated, Rabbit F (ab') 2 anti-Chicken IgG HRP conjugated antibody, Rabbit F (ab') 2 anti-Chicken IgY Antibody peroxidase Conjugated, Rabbit F (ab') 2 anti-Chicken IgY HRP conjugated antibody

Reactivity

Gallus

Immunogen

Chicken IgG whole molecule

Clonality

Polyclonal

Conjugation

HRP

Concentration

10.0 mg/mL

Dilution

ELISA: 1:40,000 - 1:160,000, IHC: 1:500 - 1:2,500, WB: 1:2,000 - 1:10,000

Purity

This product is a F (ab’) 2 fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Chicken IgG and Chicken Serum. No reaction was observed against anti-Rabbit IgG F (c) or anti-Pepsin.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

F (ab') 2 Chicken IgG Antibody Peroxidase Conjugated is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

Tested Applications

ELISA, IHC, WB

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG F (ab') 2

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