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Mouse IgG Fc Antibody

Mouse IgG F (c) antibody

Product Specifications

Product Name Alternative

Mouse IgG F (c) Antibody, Gt-a-Mouse F (c), Mouse F (c) in goat, Goat F (c) Secondary Antibody, goat anti-mouse IgG F (c) fragment antibody

Reactivity

Mouse

Immunogen

Anti-Mouse IgG was produced by repeated immunization with mouse IgG F (c) fragment in goat.

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

10.0 mg/mL

Dilution

ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000

Purity

Anti-Mouse IgG F (c) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG, Mouse IgG F (c) and Mouse Serum. No reaction was observed against Mouse IgG F (ab) .

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Mouse IgG F (c) antibody has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.

Tested Applications

ELISA, IHC, WB

Host or Source

Goat

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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