Mouse IgG Fc Antibody
Mouse IgG F (c) antibody
Product Specifications
Product Name Alternative
Mouse IgG F (c) Antibody, Gt-a-Mouse F (c), Mouse F (c) in goat, Goat F (c) Secondary Antibody, goat anti-mouse IgG F (c) fragment antibody
Reactivity
Mouse
Immunogen
Anti-Mouse IgG was produced by repeated immunization with mouse IgG F (c) fragment in goat.
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
10.0 mg/mL
Dilution
ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Purity
Anti-Mouse IgG F (c) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG, Mouse IgG F (c) and Mouse Serum. No reaction was observed against Mouse IgG F (ab) .
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Mouse IgG F (c) antibody has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
Tested Applications
ELISA, IHC, WB
Host or Source
Goat
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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