Bovine IgG F (ab') 2 Antibody Peroxidase Conjugated
Bovine IgG F (ab') 2 antibody (Peroxidase)
Product Specifications
Product Name Alternative
Rabbit anti-Bovine IgG F (ab') 2 Antibody Peroxidase Conjugated, rabbit anti-Bovine IgG Fab2 Antibody HRP Conjugated
Reactivity
Bovine
Immunogen
Bovine IgG F (ab') 2 fragment
Clonality
Polyclonal
Conjugation
HRP
Concentration
10.0 mg/mL
Dilution
ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
Purity
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Bovine IgG, Bovine IgG F (ab’) 2 and Bovine Serum. No reaction was observed against Bovine IgG F (c) .
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Tested Applications
ELISA, IHC, WB
Host or Source
Rabbit
Preservative
Preservative: None. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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