Mouse IgG3 isotype control Phycoerythrin Antibody
Mouse IgG3 isotype control Phycoerythrin Antibody
Product Specifications
Product Name Alternative
Phycoerythrin conjugated Mouse IgG3 isotype control, Mouse IgG3 subclass isotype control PE conjugation
Clone
MG3
Conjugation
RPE
Source
Mouse
Field of Research
Immunology & Inflammation
Concentration
1.0 mg/mL
Dilution
FC: 1:1000-1:5000, IF: 1:1000-1:5000
Purity
This product was prepared from ascitic fluid by affinity chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG3 and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgA, IgM. Phycoerythrin Conjugated Mouse IgG3 was proven by Dot Blot.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent) . This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
Notes
For research use only.
Applications Notes
Mouse IgG3 isotype control can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
Tested Applications
FC, IF
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG3
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