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HDAC4 Antibody

HDAC-4 antibody

Product Specifications

Product Name Alternative

Rabbit anti-HDAC-4 antibody, HDAC4, HDAC 4, HD 4 antibody, histone deacetylase 4 antibody, Histone deacetylase-4, HD4 antibody

UniProt

P56524

Reactivity

Human, Mouse, Primate, Rat

Immunogen

HDAC4 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region surrounding 600-650aa of human HDAC4.

Target

HDAC4

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Epigenetics & Chromatin

Concentration

1 mg/mL

Dilution

ELISA: 1:20,000 - 1:60,000, IHC: 1 mg/mL, IF: 1:100-1:500, WB: 1 mg/mL

Purity

Anti-HDAC4 was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific towards HDAC4. A BLAST analysis was used to suggest cross-reactivity with Human, Mouse, Rat, and Primate based on 100% sequence homology. Cross-reactivity with HDAC4 from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-HDAC4 antibody has been tested in Western Blot and is useful for ELISA and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~140 kDa corresponding to the appropriate cell lysate or extract.

Tested Applications

ELISA, IF, IHC, WB

NCBI Accession Number

NP_006028.2

Host or Source

Rabbit

Preservative

Preservative: 50% (v/v) Glycerol; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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