GAB1 Antibody

Rabbit anti-GAB1 pY659 antibody, GAB-1, GAB 1, GRB2-associated-binding protein 1, GRB2-associated binder 1, Growth factor receptor bound protein 2-associated protein 1

Anti-GAB1 pY656 Antibody was produced in rabbits by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y659 of human GAB1 protein.

Anti-GAB1pY659 was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorptions against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Antibody is specific for human GAB1 protein phosphorylated at Y659. Cross-reactivity against GAB1 from other species may occur but has not yet been tested.

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-GAB1 pY656 Antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at 76.6 kDa by western blotting in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with human GAB1 pY659 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.