SMAD1 Antibody

Rabbit anti-SMAD1 pS206 antibody, SMAD-1, SMAD 1, mothers against decapentaplegic homolog 1 antibody, MAD homolog 1, Mothers against DPP homolog 1, SMAD family member 1, MADH1, MADH 1, Transforming growth factor-beta-signaling protein 1, BSP-1, JV4-1

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a phosphorylated synthetic peptide corresponding to the region of amino acids containing serine 206 of human SMAD1 protein.

This affinity-purified antibody is directed against the phosphorylated form of human SMAD1 protein at the pS206 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human SMAD1 pS206 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human SMAD1 is minimal by ELISA and western blot. A BLAST analysis was used to suggest 100% cross reactivity with SMAD1 from human, Duckbill platypus, Drosophila, mouse, opossum, chimpanzee, bonobo, orangutan, gorilla, macaque, cattle, sheep, pig, rat, horse, salmon, chicken, and dog based on the sequence homology with the immunogen. Reactivity against homologues from other sources is not known.

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 52 kDa in size corresponding to phosphorylated SMAD1 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. This antibody is specific for the phosphorylated pS206 of SMAD1. Stimulation of EGF, TGFbeta BMP2, and 0.5M NaCl are recommended for 1 hour.