Biotin Antibody Peroxidase Conjugated
Biotin antibody (Peroxidase)
Product Specifications
Product Name Alternative
Goat anti-Biotin Antibody HRP Conjugation, Peroxidase conjugated goat anti-Biotin Antibody
Immunogen
Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
Clonality
Polyclonal
Conjugation
HRP
Concentration
1.0 mg/mL
Dilution
ELISA: 1:140,000, IHC: 1:1,000 - 1:5,000, WB: 1:3,000 - 1:15,000
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, biotinylated bovine serum albumin and Biotinylated IgG.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Biotin Peroxidase conjugated antibody has been tested by ELISA and dot blot. This product is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Tested Applications
DOT, ELISA, IHC, WB
Host or Source
Goat
Preservative
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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