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Il17a Antibody (Biotin)

IL-17A antibody (Biotin)

Product Specifications

Product Name Alternative

Rabbit anti-IL-17A biotin Conjugated Antibody, rabbit anti-Interleukin-17A biotin Conjugated Antibody, Interleukin17, IL-17, Cytotoxic T-lymphocyte-associated antigen 8 and CTLA-8

UniProt

Q61453

Reactivity

Mouse, Rat

Immunogen

This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant rat IL17-A protein.

Target

Il17a

Clonality

Polyclonal

Conjugation

Biotin

Field of Research

Immunology & Inflammation

Concentration

1.0 mg/mL

Dilution

ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000

Purity

This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant rat IL17-A in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This purified antibody has been tested for use in western blotting and suitable in ELISA. By western blot a band approximately 30.0 kDa in size corresponding to rat IL17-A protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

Q61453.1

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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