Ccl20 Antibody (Biotin)
MIP-3 alpha antibody (Biotin)
Product Specifications
Product Name Alternative
Rabbit anti-MIP-3α biotin conjugated antibody, CCL20, C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1
UniProt
O89093
Reactivity
Mouse, Rat
Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse MIP-3α protein.
Target
Ccl20
Clonality
Polyclonal
Conjugation
Biotin
Field of Research
Immunology & Inflammation
Concentration
1.0 mg/mL
Dilution
ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
Purity
This Protein A purified antibody was heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant mouse MIP-3α in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This purified antibody has been tested by dot blot and is suitable for use in ELISA and western blotting. By western blot a band approximately 10.7 kDa in size corresponding to mouse MIP-3α protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Tested Applications
DOT, ELISA, IHC, WB
NCBI Accession Number
NP_058656.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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