Gox Antibody (HRP)
Glucose Oxidase antibody (Peroxidase)
Product Specifications
Product Name Alternative
Rabbit anti-Glucose Oxidase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-Glucose Oxidase Antibody, Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody
UniProt
P13006
Reactivity
Fungi
Immunogen
Glucose Oxidase [Aspergillus niger]
Target
Gox
Clonality
Polyclonal
Conjugation
HRP
Field of Research
Metabolism Research
Concentration
1.0 mg/mL
Dilution
ELISA: 1:5,000 - 1:25,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
Purity
Glucose Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Glucose Oxidase [Aspergillus niger]. Cross reactivity against Glucose Oxidase from other tissues and species may occur but has not been specifically determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-Glucose Oxidase Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is useful in Immunohistochemistry. Optimal titers should be obtained by researcher.
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
P13006.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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